Forward and reverse mutagenesis in C. elegans.
نویسندگان
چکیده
Mutagenesis drives natural selection. In the lab, mutations allow gene function to be deciphered. C. elegans is highly amendable to functional genetics because of its short generation time, ease of use, and wealth of available gene-alteration techniques. Here we provide an overview of historical and contemporary methods for mutagenesis in C. elegans, and discuss principles and strategies for forward (genome-wide mutagenesis) and reverse (target-selected and gene-specific mutagenesis) genetic studies in this animal.
منابع مشابه
Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software
Cellular processes, such as chromosome assembly, segregation and cytokinesis,are inherently dynamic. Time-lapse imaging of living cells, using fluorescent-labeled reporter proteins or differential interference contrast (DIC) microscopy, allows for the examination of the temporal progression of these dynamic events which is otherwise inferred from analysis of fixed samples(1,2). Moreover, the st...
متن کاملSynaptic function.
C. elegans has emerged as a powerful genetic model organism in which to study synaptic function. Most synaptic proteins in the C. elegans genome are highly conserved and mutants can be readily generated by forward and reverse genetics. Most C. elegans synaptic protein mutants are viable affording an opportunity to study the functional consequences in vivo. Recent advances in electrophysiologica...
متن کاملGenetics in Invertebrates: Modeling Dopaminergic Signaling and Neurodegeneration
INTRODUCTION From worms to humans, dopamine(DA) is one of the most important neurotransmitters in regulating complex definitive behaviors. In humans, DA’s actions extend to processes like arousal, learning, motor control and reward, the defects of which are evident in disorders such as ADHD, PD, bipolar disorder, and schizophrenia. Our understanding of these processes and disease states has adv...
متن کاملComparative genetics of sex determination: masculinizing mutations in Caenorhabditis briggsae.
The nematodes Caenorhabditis elegans and C. briggsae independently evolved self-fertile hermaphroditism from gonochoristic ancestors. C. briggsae has variably divergent orthologs of nearly all genes in the C. elegans sex determination pathway. Their functional characterization has generally relied on reverse genetic approaches, such as RNA interference and cross-species transgene rescue and mor...
متن کاملReverse Genetic Strategies in Caenorhabditis elegans: Towards Controlled Manipulation of the Genome
Caenorhabditis elegans has a complete annotated genome sequence that is augmented by increasing quantities of data from high-throughput postgenomic analyses. This has led to an increasing need to identify the biological functions of specific genes using reverse genetics, i.e., moving from gene to phenotype. Fundamental to this aim is the ability to alter the structure of particular genes by mea...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- WormBook : the online review of C. elegans biology
دوره شماره
صفحات -
تاریخ انتشار 2014